Evaluation of different disinfection protocols for seed germination  of Ocimum basilicum L.

William  Gallego Idárraga; Santiago  Herrada Chávez; Karol Andrea Leal Vásquez; Valentina  Lamus Molina; Luisa Fernanda  Cabezas Burbano

doi:10.54502/msuceva.v3n2a8

Autores/as

William Gallego Idárraga Unidad Central del Valle del Cauca-UCEVA. Carrera 27A No. 48 -144 Kilometre 1 South Exit. - Tuluá - Valle del Cauca - Colombia. Postal Code: 763022.
Santiago Herrada Chávez Unidad Central del Valle del Cauca-UCEVA. Carrera 27A No. 48 -144 Kilometre 1 South Exit. - Tuluá - Valle del Cauca - Colombia. Postal Code: 763022.
Karol Andrea Leal Vásquez PROAGRO Research Group. Unidad Central del Valle del Cauca-UCEVA. Carrera 27A No. 48 -144 Kilometre 1 South Exit. - Tuluá - Valle del Cauca - Colombia. Postal Code: 763022.
Valentina Lamus Molina TOLUES & PROAGRO Research Group. Unidad Central del Valle del Cauca-UCEVA. Carrera 27A No. 48 -144 Kilometre 1 South Exit. - Tuluá - Valle del Cauca - Colombia. Postal Code: 763022.
Luisa Fernanda Cabezas Burbano ENERGIAS Research Group. Unidad Central del Valle del Cauca-UCEVA. Carrera 27A No. 48 -144 Kilometre 1 South Exit. - Tuluá - Valle del Cauca - Colombia. Postal Code: 763022.

DOI:

https://doi.org/10.54502/msuceva.v3n2a8

Palabras clave:

Aromatic plant, basil, dormancy, in vitro culture, micropropagation

Resumen

The aim of this study was to discriminate between different disinfection protocols applied to common basil seeds for in vitro establishment on Murashige & Skoog medium. The aim of this approach was not only to overcome seed dormancy, but also to decisively influence the germination process in order to achieve a significant increase in the proportion of seeds that germinate successfully. In the Colombian context, where some 156 species of medicinal plants are currently marketed, basil (Ocimum basilicum L.), originally from Asia Minor, stands out as one of the most widely used plants in the world. In the department of Valle del Cauca, medicinal plants have emerged as protagonists in contrast to the monoculture of sugar cane (Saccharum Officinarum L.) that dominates the region. However, basil faces significant challenges related to seed dormancy, a condition that negatively affects germination and therefore directly affects yield. In this scenario, 21 treatments were carried out in the Biological Sciences Laboratory of the Central Unit of Valle del Cauca, which showed a marked variability and influence in phenological development, especially in stem and leaf growth. Treatment 12 (0.7% NaClO) showed the highest percentage of basil seed germination (80%). It is imperative to explore new protocols that effectively contribute to the basil production process by overcoming seed dormancy.

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Biografía del autor/a

William Gallego Idárraga , Unidad Central del Valle del Cauca-UCEVA. Carrera 27A No. 48 -144 Kilometre 1 South Exit. - Tuluá - Valle del Cauca - Colombia. Postal Code: 763022.

Agricultural Engineer from the Unidad Central Unit del Valle del Cauca and Agricultural Business Technologist. He has worked in animal health, teaching, at the Ministry of Agriculture and Development in Buga municipality of Valle del Cauca and at the Colombian Agricultural Institute (ICA).

Santiago Herrada Chávez, Unidad Central del Valle del Cauca-UCEVA. Carrera 27A No. 48 -144 Kilometre 1 South Exit. - Tuluá - Valle del Cauca - Colombia. Postal Code: 763022.

Agricultural engineer from the Central Unit of Valle del Cauca, with the ability to design, project and improve agricultural production systems and/or processes, adapting easily to any environment and using the most modern methods. He has worked in animal health, teaching, the Ministry of Agriculture and Development of Buga municipality and the Colombian Agricultural Institute (ICA).

Karol Andrea Leal Vásquez, PROAGRO Research Group. Unidad Central del Valle del Cauca-UCEVA. Carrera 27A No. 48 -144 Kilometre 1 South Exit. - Tuluá - Valle del Cauca - Colombia. Postal Code: 763022.

Agricultural Engineering from the National University of Colombia and a Master's Degree in Agricultural Sciences with a specialisation in Plant Breeding from the Palmira Campus of the National University of Colombia. She is currently attached to the Central Unit of Valle del Cauca, in Tuluá. As a full-time teacher. With 11 years of professional experience. She has worked as an agricultural consultant in the municipality of Cundinamarca. She is currently linked to the PROAGRO and Genetic Improvement, Agronomy and Vegetable Seed Production research groups.

Valentina Lamus Molina, TOLUES & PROAGRO Research Group. Unidad Central del Valle del Cauca-UCEVA. Carrera 27A No. 48 -144 Kilometre 1 South Exit. - Tuluá - Valle del Cauca - Colombia. Postal Code: 763022.

Biologist from the University of Antioquia-UDEA, Colombia; she holds an M.Sc. and Ph.D. in Agricultural Sciences and Natural Resources from the Autonomous University of the State of Mexico, Mexico. Her research area is in the field of mycology, with special emphasis on the taxonomy of mycorrhizal fungi and their interaction with plants of forestry and agricultural interest. She currently works as a teacher of Biology and Phytopathology in the Agricultural Engineering Programme of the Unidad Central del Valle del Cauca, she is also currently part of the TOLUES research group in the Environmental Engineering programme, where she leads the Fungal Connections subgroup, which focuses on the study of fungi and their various applications.

Luisa Fernanda Cabezas Burbano, ENERGIAS Research Group. Unidad Central del Valle del Cauca-UCEVA. Carrera 27A No. 48 -144 Kilometre 1 South Exit. - Tuluá - Valle del Cauca - Colombia. Postal Code: 763022.

She has a degree in Physical Engineering from the University of Cauca and a PhD in Physical Sciences from the National University of La Plata, Argentina. She is currently attached to the Central Unit of Valle del Cauca as a full-time teacher. She has 12 years of professional experience. She belongs to the Energy Research Group, attached to the Faculty of Engineering. Her area of performance is basic sciences as a significant contribution to the agricultural sector.

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